VirusTotal inspects items with over 70 antivirus scanners and URL/domain blacklisting services, in addition to a myriad of tools to extract signals from the studied content. Any user can select a file from their computer using their browser and send it to VirusTotal. VirusTotal offers a number of file submission methods, including the primary public web interface, desktop uploaders, browser extensions and a programmatic API. The web interface has the highest scanning priority among the publicly available submission methods. Submissions may be scripted in any programming language using the HTTP-based public API.

As with files, URLs can be submitted via several different means including the VirusTotal webpage, browser extensions and the API.

Upon submitting a file or URL basic results are shared with the submitter, and also between the examining partners, who use results to improve their own systems. As a result, by submitting files, URLs, domains, etc. to VirusTotal you are contributing to raise the global IT security level.